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KMID : 0881720190340050431
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Journal of Food Hygiene and Safety
2019 Volume.34 No. 5 p.431 ~ p.437
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Development of Simultaneous Analysis of Ferulic Acid, Caffeic Acid, Catechin and Taxifolin from Health Functional Food Pinus Pinaster Bark Extract by UPLC-MS/MS
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Oh Jae-Myoung
Kim Ji-An
Hu Soo-Jung
Choi Yoon-Hee
Oh Keum-Soon
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Abstract
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This study was conducted to develop a simultaneous analysis method for ferulic acid, caffeic acid, catechin and taxifolin from Health Functional Food (HFF) Pinus Pinaster bark extract. The simultaneous analytical method for ferulic acid, caffeic acid, catechin and taxifolin is carried out using UPLC-MS/MS. The method validation was performed to determine selectivity, linearity, accuracy, limit of detection (LOD), limit of quantification (LOQ) and precision for ferulic acid, caffeic acid, catechin and taxifolin. LC-MS/MS method was established using an Acquity UPLC BEH C18 Column and was applied for these 4 compounds. Product-ion traces, at m/z 194.2 → 133, 180.2 → 135, 290.3 → 245, 304.3 → 248, were used for quantitative analysis of ferulic acid, caffeic acid, catechin and taxifolin, respectively. Excellent linearity (r2=0.999) was observed for ferulic acid, caffeic acid, catechin and taxifolin in the concentration range (50-2500 mg/L). The observed recoveries of these 4 compounds were found to be between 84.9 and 104.9%, while precision was between 1.20 and 4.43% relative standard deviation (% RSD).
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KEYWORD
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Health functional food, Pinus Pinaster Bark, Ferulic acid, Caffeic acid, Catechin, Taxifolin
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